| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Farney, Olivia J (15)
|
ADGUY |
29.06 |
28.99 |
-0.07 |
| 2 |
Redick, Nathalie R (15)
|
ADSARY |
28.54 |
29.04 |
0.50 |
| 3 |
Frenyea, Sophia J (15)
|
ADGLOY |
28.74 |
29.20 |
0.46 |
| 4 |
Koeferl, Alexandra M (15)
|
ADGGFY |
27.84 |
29.22 |
1.38 |
| 5 |
Reynolds, Reaghan E (15)
|
ADDACC |
27.84 |
29.30 |
1.46 |
| 6 |
Willis, Isabelle J (15)
|
ADGLOY |
31.52 |
29.40 |
-2.12 |
| 7 |
O'Loughlin, Natalie G (15)
|
ADSSCY |
28.57 |
29.58 |
1.01 |
| 8 |
Cromer, Olivia A (16)
|
ADGLOY |
29.50 |
30.56 |
1.06 |
| 9 |
Felton, Kristin M (16)
|
ADGLFY |
29.18 |
31.30 |
2.12 |
| 10 |
Ormsby, Lela (15)
|
ADGGFY |
33.45 |
31.31 |
-2.14 |
| 11 |
Austin, Skylar (15)
|
ADGGFY |
|
33.73 |
- |